Production of Foot-and-Mouth Disease Type O and A Vaccine Antigens on a Pilot Scale and Determination of Optimal Amount of Antigen for Monovalent Vaccines

Foot-and-mouth disease (FMD) is a highly infectious affecting cloven-hoofed animals and causes significant economic losses to the livestock industry. The Type O PanAsia-2 (O PA-2) vaccine strain protective against wide range of serotype FMD virus (FMDV) strains in East Asia, A22 Iraq/24/64 (A22 IRQ) most widely used antigen banks. aim this study was produce antigens from PA-2 IRQ viruses using 100 L bioreactor evaluate efficacy varying concentrations pigs. More than 2 μg/mL recovered virus-infected supernatants. Further, inactivation by binary ethyleneimine revealed that viral titers decreased below 10−7 TCID50/mL within 13 h 9 h, respectively. vaccines, containing 10 μg 5 antigen, respectively, provided protection homologous This first report demonstrating obtained pilot-scale production are viable candidate vaccines. These results will pave way for industrial-scale South Korea.